Chemical constituents of the volatile oil from leaves of Annona coriacea and in vitro antiprotozoal activity

The essential oil of the leaves from Annona coriacea Mart., Annonaceae, was extracted by hydrodistillation in a Clevenger apparatus and analyzed by GC/MS and GC/FID. The oil yield was 0.05% m/m. Sixty compounds were identified, in a complex mixture of sesquiterpenes (76.7%), monoterpenes (20.0%) and other constituents (3.3%). Bicyclogermacrene was its major compound (39.8%) followed by other sesquiterpenes. Most of the monoterpenes were in low concentration (<1%). Only &#946;-pinene and pseudolimonene presented the highest level of 1.6%. The volatile oil presented anti-leishmanial and trypanocidal activity against promastigotes of four species of Leishmania and trypomastigotes of Trypanosoma cruzi, showing to be more active against Leishmania (L.) chagasi (IC50 39.93 µ g/mL) (95% CI 28.00-56.95 µ g/mL)

Chemical constituents of the volatile oil from leaves of Annona coriacea and in vitro antiprotozoal activity

The essential oil of the leaves from Annona coriacea Mart., Annonaceae, was extracted by hydrodistillation in a Clevenger apparatus and analyzed by GC/MS and GC/FID. The oil yield was 0.05% m/m. Sixty compounds were identified, in a complex mixture of sesquiterpenes (76.7%), monoterpenes (20.0%) and other constituents (3.3%). Bicyclogermacrene was its major compound (39.8%) followed by other sesquiterpenes. Most of the monoterpenes were in low concentration (<1%). Only &#946;-pinene and pseudolimonene presented the highest level of 1.6%. The volatile oil presented anti-leishmanial and trypanocidal activity against promastigotes of four species of Leishmania and trypomastigotes of Trypanosoma cruzi, showing to be more active against Leishmania (L.) chagasi (IC50 39.93 µ g/mL) (95% CI 28.00-56.95 µ g/mL).21

Chemical constituents of the volatile oil from leaves of annona coriacea and in vitro antiprotozoal activity

The essential oil of the leaves from Annona coriacea Mart., Annonaceae, was extracted by hydrodistillation in a Clevenger apparatus and analyzed by GC/MS and GC/FID. The oil yield was 0.05% m/m. Sixty compounds were identified, in a complex mixture of sesquiterpenes (76.7%), monoterpenes (20.0%) and other constituents (3.3%). Bicyclogermacrene was its major compound (39.8%) followed by other sesquiterpenes. Most of the monoterpenes were in low concentration (<1%). Only β-pinene and pseudolimonene presented the highest level of 1.6%. The volatile oil presented anti-leishmanial and trypanocidal activity against promastigotes of four species of Leishmania and trypomastigotes of Trypanosoma cruzi, showing to be more active against Leishmania (L.) chagasi (IC50 39.93 µ g/mL) (95% CI 28.00-56.95 µ g/mL)2113340COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informaçãoSem informaçã

Anticholinesterase activity evaluation of alkaloids and coumarin from stems of Conchocarpus fontanesianus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conchocarpus fontanesianus (A. St.-Hill.) Kallunki & Pirani, Rutaceae, popularly known as pitaguara, is a native and endemic tree from Sao Paulo and Rio de Janeiro States, Brazil. Based in the information that anticholinesterasic derivatives could act as new prototypes to treatment of Alzheimer disease, this work describes the fractionation guided by evaluation of the anticholinesterase activity of the ethanolic stems extract from C. fontanesianus. This procedure afforded the alkaloids dictamnine (1), gamma-fagarine (2), skimianine (3), and 2-phenyl-1-methyl-4-quinolone (4), as well as the coumarin marmesin (5).222374380Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Extraction of phenolic compounds from pepper-rosmarin (lippia sidoides cham.) leaves by sequential extraction in fixed bed extractor using supercritical CO2, ethanol and water as solvents

Extraction of bioactive compounds requires frequent changes in the extraction techniques due to the quality requirements in the food, pharmaceutical and cosmetic industries. The compounds of the polyphenolic family represent an important group of bioactive compounds in food, and are present in various types of plants, including Lippia sidoides Cham., also known as pepper-rosmarin, an aromatic plant with therapeutic properties. Thus, this study aimed to investigate the sequential extraction (in fixed bed and high pressure) of bioactive compounds from L. sidoides leaves, using supercritical carbon dioxide, ethanol, and water as solvents and, compare the global yield, total phenolics, total flavonoids, and antioxidant activity of extracts obtained from sequential extraction with the extracts obtained by one-step extraction in fixed bed (high pressure) and by conventional extraction. The results showed that the combination of extraction processes is an effective method to obtain extracts with different compositions and functionalities. Overall, the ethanolic extraction in fixed bed and the three-step sequential extraction showed the best results in terms of global yield, and content and yield of total phenolics and flavonoids. The ethanol extracts exhibited the best antioxidant activity, which may be related to the presence of phenolic compounds996875CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informação2011/14309-

Production of copaiba oleoresin particles from emulsions stabilized with modified starches

The oleoresin of copaiba has many pharmacological properties, such as anti-inflammatory, antiseptic, and cicatrizing, besides helping to treat skin diseases, among other applications. In order to improve its activity, copaiba oleoresin was encapsulated within polymeric particles through emulsification assisted by ultrasound followed by a drying process. Ultrasound-assisted emulsification led to the formation of kinetically stable emulsions. The emulsions with increased stability and the lowest droplet diameters (80.95 ± 2.1 nm (Hi-Cap 100®) and 71.8 ± 2.9 nm (Snow-Flake® E 6131)) were subjected to freeze-drying and spray-drying to produce dry particles. The particles were characterized in terms of moisture content, particle size distribution, encapsulation efficiency, surface oil, oxidative stability, X-ray diffraction and morphological analysis by FESEM and CLSM. Both drying techniques produced amorphous particles with different sizes and low moisture content. Furthermore, high encapsulation efficiency and high stability against thermal degradation were achieved. The analysis of the particles’ morphology indicated that the nature of the biopolymers used (Hi-Cap 100® and Snow-Flake® E 6131) did not affect their microstructure. However, as expected, the freeze-drying and spray-drying techniques produced particles with different external microstructures. SD particles were spherical, whereas FD particles presented irregular structures similar to sheets108128139CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP472523/2013-9Sem informação2015/11932-7; 2016/13602-

Comparison of the chemical composition of the essential oil and the water soluble oil of baccharis dracunculifolia DC. (asteraceae)

This study aimed to evaluate the chemical composition of Baccharis dracunculifolia essential oil and the water soluble oil obtained by steam distillation that were analyzed by GC and GC/MS. In the first hour of distillation B. dracunculifolia aerial parts yielded 0.08% oil and in the second hour, 0.27%. The oil recovered from the distillate water yielded 0.18 g/L in the first hour and 0.44 g/L in the second hour of distillation. The main volatile compounds identified in the distillate water were aromatic compounds and sesquiterpene alcohols202111114FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP01/14219-7; 04/08756-

Comparison of the chemical composition of the essential oil and the water soluble oil of Baccharis dracunculifolia DC. (Asteraceae)

This study aimed to evaluate the chemical composition of Baccharis dracunculifolia essential oil and the water soluble oil obtained by steam distillation that were analyzed by GC and GUMS. in the first hour of distillation, B. dracunculifolia aerial parts yielded 0.08% oil and in the second hour, 0.27%. The oil recovered from the distillate water yielded 0.18 g/L in the first hour and 0.44 g/L in the second hour of distillation. The main volatile compounds identified in the distillate water were aromatic compounds and sesquiterpene alcohols.20211111

Comparison of the chemical composition of the essential oil and the water soluble oil of Baccharis dracunculifolia DC. (Asteraceae)

This study aimed to evaluate the chemical composition of Baccharis dracunculifolia essential oil and the water soluble oil obtained by steam distillation that were analyzed by GC and GUMS. in the first hour of distillation, B. dracunculifolia aerial parts yielded 0.08% oil and in the second hour, 0.27%. The oil recovered from the distillate water yielded 0.18 g/L in the first hour and 0.44 g/L in the second hour of distillation. The main volatile compounds identified in the distillate water were aromatic compounds and sesquiterpene alcohols